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KMID : 0380620100420030329
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Korean Journal of Food Science and Technology
2010 Volume.42 No. 3 p.329 ~ p.334
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Production of Monoclonal Antibody against Escherichia coli O157:H7 and Development of Enzyme Linked Immunosorbent Assay
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Ryu Hee-Jeong
Kim Jung-Sook
Kim Kyeong-Yeol
Nam Bo-Ram
Nam Min-Ji
Shim Won-Bo
Kim Nam-Soo
Cho Yong-Jin
Chung Duck-Hwa
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Abstract
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Escherichia coli O157:H7 causes hemolytic uremic syndrome and hemorrhagic colitis in humans. The objectives of this study were to produce monoclonal antibody(MAb) against E. coli O157:H7 and to develop an enzyme linked immunosorbent assay(ELISA) for the rapid detection of E. coli O157:H7 in agri-stockbreeding. The characterization of MAb produced from hybridoma cell (HKEC 4G8-5) was validated by ELISA and Western blot. The produced MAb was specific to E. coli O157:H7 and showed weak cross-reaction to Staphylococcus aureus. The detection limit of ELISA based on 4G8-5 MAb was 105 cell/mL. Although the ELISA could not detect E. coli O157:H7 in the meat and sprout samples inoculated with 1×101 cell/10 g without enrichment, the same samples after enrichment for 6 hr were confirmed to be positive by ELISA. These results indicated that the ELISA combined with short enrichment (6 hr) is useful tool for rapid screening of E. coli O157:H7 in various samples.
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KEYWORD
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E. coli O157:H7, monoclonal antibody, ELISA
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